node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
ACS39514.1 | rutA | MexAM1_META1p1665 | MexAM1_META1p1664 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | 0.626 |
ACS39514.1 | rutB | MexAM1_META1p1665 | MexAM1_META1p1663 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.592 |
ACS39514.1 | rutC | MexAM1_META1p1665 | MexAM1_META1p1662 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.522 |
ACS39514.1 | rutD | MexAM1_META1p1665 | MexAM1_META1p1661 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Putative enzyme of alternative pyrimidine degradation pathway, putative alpha/beta hydrolase; May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation. Belongs to the AB hydrolase superfamily. Hydrolase RutD family. | 0.577 |
ACS39514.1 | rutF | MexAM1_META1p1665 | MexAM1_META1p1660 | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | Flavin reductase domain protein, FMN-binding; Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway; Belongs to the non-flavoprotein flavin reductase family. RutF subfamily. | 0.547 |
ACS41011.1 | ACS42040.1 | MexAM1_META1p3269 | MexAM1_META1p4409 | Hypothetical protein; Similar to elongation factor G (C-terminus); Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative factor. | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | 0.464 |
ACS41011.1 | rutC | MexAM1_META1p3269 | MexAM1_META1p1662 | Hypothetical protein; Similar to elongation factor G (C-terminus); Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative factor. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.464 |
ACS42040.1 | ACS41011.1 | MexAM1_META1p4409 | MexAM1_META1p3269 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Hypothetical protein; Similar to elongation factor G (C-terminus); Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative factor. | 0.464 |
ACS42040.1 | fusA | MexAM1_META1p4409 | MexAM1_META1p2150 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Protein chain elongation factor EF-G, GTP-binding; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase f [...] | 0.464 |
ACS42040.1 | ilvA | MexAM1_META1p4409 | MexAM1_META1p3241 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Threonine deaminase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.406 |
ACS42040.1 | rph | MexAM1_META1p4409 | MexAM1_META1p0317 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Ribonuclease PH (RNase PH) (tRNA nucleotidyltransferase); Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. | 0.499 |
ACS42040.1 | rutC | MexAM1_META1p4409 | MexAM1_META1p1662 | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.735 |
fusA | ACS42040.1 | MexAM1_META1p2150 | MexAM1_META1p4409 | Protein chain elongation factor EF-G, GTP-binding; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase f [...] | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | 0.464 |
fusA | rutC | MexAM1_META1p2150 | MexAM1_META1p1662 | Protein chain elongation factor EF-G, GTP-binding; Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome; Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase f [...] | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.464 |
ilvA | ACS42040.1 | MexAM1_META1p3241 | MexAM1_META1p4409 | Threonine deaminase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | 0.406 |
ilvA | rutC | MexAM1_META1p3241 | MexAM1_META1p1662 | Threonine deaminase; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.524 |
rph | ACS42040.1 | MexAM1_META1p0317 | MexAM1_META1p4409 | Ribonuclease PH (RNase PH) (tRNA nucleotidyltransferase); Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. | Conserved hypothetical protein; Homologs of previously reported genes of unknown function. | 0.499 |
rph | rutC | MexAM1_META1p0317 | MexAM1_META1p1662 | Ribonuclease PH (RNase PH) (tRNA nucleotidyltransferase); Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. | Enzyme of alternative pyrimidine degradation pathway; May reduce aminoacrylate peracid to aminoacrylate. Required to remove a toxic intermediate produce by the pyrimidine nitrogen degradation. | 0.535 |
rutA | ACS39514.1 | MexAM1_META1p1664 | MexAM1_META1p1665 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Putative transcriptional regulator, TetR/AcrR family; Function proposed based on presence of conserved amino acid motif, structural feature or limited homology; putative regulator. | 0.626 |
rutA | rutB | MexAM1_META1p1664 | MexAM1_META1p1663 | Putative luciferase family protein, putative flavin-dependent oxidoreductase; Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product which can be spontaneously reduced to ureidoacrylate. | Enzyme of alternative pyrimidine degradation pathway; In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2; Belongs to the isochorismatase family. RutB subfamily. | 0.995 |