node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
PA0851 | PA1417 | PA0851 | PA1417 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | 0.966 |
PA0851 | ilvA1 | PA0851 | PA0331 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Threonine dehydratase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.923 |
PA0851 | ilvA2 | PA0851 | PA1326 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Threonine dehydratase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.922 |
PA0851 | ilvC | PA0851 | PA4694 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.725 |
PA0851 | ilvH | PA0851 | PA4695 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Acetolactate synthase isozyme III small subunit; Product name confidence: Class 2 (High similarity to functionally studied protein). | 0.981 |
PA0851 | ilvI | PA0851 | PA4696 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | Acetolactate synthase large subunit; Product name confidence: Class 2 (High similarity to functionally studied protein). | 0.970 |
PA0851 | leuB | PA0851 | PA3118 | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate; Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.940 |
PA1417 | PA0851 | PA1417 | PA0851 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Hypothetical protein; Product name confidence: Class 4 (Homologs of previously reported genes of unknown function, or no similarity to any previously reported sequences). | 0.966 |
PA1417 | PA2108 | PA1417 | PA2108 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | 0.882 |
PA1417 | ilvA1 | PA1417 | PA0331 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Threonine dehydratase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.942 |
PA1417 | ilvA2 | PA1417 | PA1326 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Threonine dehydratase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.943 |
PA1417 | ilvC | PA1417 | PA4694 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.983 |
PA1417 | ilvH | PA1417 | PA4695 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Acetolactate synthase isozyme III small subunit; Product name confidence: Class 2 (High similarity to functionally studied protein). | 0.970 |
PA1417 | ilvI | PA1417 | PA4696 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Acetolactate synthase large subunit; Product name confidence: Class 2 (High similarity to functionally studied protein). | 0.692 |
PA1417 | leuA | PA1417 | PA3792 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.894 |
PA1417 | leuB | PA1417 | PA3118 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate; Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.968 |
PA1417 | poxB | PA1417 | PA5297 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Pyruvate dehydrogenase (cytochrome); Product name confidence: Class 2 (High similarity to functionally studied protein); Belongs to the TPP enzyme family. | 0.863 |
PA2108 | PA1417 | PA2108 | PA1417 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | 0.882 |
PA2108 | ilvC | PA2108 | PA4694 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.726 |
PA2108 | ilvH | PA2108 | PA4695 | Probable decarboxylase; Product name confidence: Class 3 (Function proposed based on presence of conserved amino acid motif, structural feature or limited sequence similarity to an experimentally studied gene); Belongs to the TPP enzyme family. | Acetolactate synthase isozyme III small subunit; Product name confidence: Class 2 (High similarity to functionally studied protein). | 0.969 |