node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
PFL_2651 | PFL_4812 | PFL_2651 | PFL_4812 | HpcH/HpaI aldolase/citrate lyase family protein; Identified by match to protein family HMM PF03328; Belongs to the HpcH/HpaI aldolase family. | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | 0.900 |
PFL_3440 | PFL_4812 | PFL_3440 | PFL_4812 | Identified by match to protein family HMM PF00291. | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | 0.935 |
PFL_3440 | hom | PFL_3440 | PFL_1103 | Identified by match to protein family HMM PF00291. | Homoserine dehydrogenase; Identified by similarity to SP:P29365; match to protein family HMM PF00742; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.872 |
PFL_3440 | ilvA_1 | PFL_3440 | PFL_3098 | Identified by match to protein family HMM PF00291. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.926 |
PFL_3440 | ilvA_2 | PFL_3440 | PFL_5905 | Identified by match to protein family HMM PF00291. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.924 |
PFL_3440 | ilvC | PFL_3440 | PFL_5253 | Identified by match to protein family HMM PF00291. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.640 |
PFL_3440 | leuB | PFL_3440 | PFL_2066 | Identified by match to protein family HMM PF00291. | 3-isopropylmalate dehydrogenase; Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. | 0.949 |
PFL_3649 | PFL_4812 | PFL_3649 | PFL_4812 | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | 0.950 |
PFL_3649 | hom | PFL_3649 | PFL_1103 | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | Homoserine dehydrogenase; Identified by similarity to SP:P29365; match to protein family HMM PF00742; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.903 |
PFL_3649 | ilvC | PFL_3649 | PFL_5253 | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.870 |
PFL_3649 | leuA | PFL_3649 | PFL_4946 | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.746 |
PFL_3649 | leuA-2 | PFL_3649 | PFL_4123 | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | Putative 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.750 |
PFL_4812 | PFL_2651 | PFL_4812 | PFL_2651 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | HpcH/HpaI aldolase/citrate lyase family protein; Identified by match to protein family HMM PF03328; Belongs to the HpcH/HpaI aldolase family. | 0.900 |
PFL_4812 | PFL_3440 | PFL_4812 | PFL_3440 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Identified by match to protein family HMM PF00291. | 0.935 |
PFL_4812 | PFL_3649 | PFL_4812 | PFL_3649 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Tartrate dehydrogenase; Identified by similarity to SP:P76251; match to protein family HMM PF00180; match to protein family HMM TIGR02089. | 0.950 |
PFL_4812 | hom | PFL_4812 | PFL_1103 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Homoserine dehydrogenase; Identified by similarity to SP:P29365; match to protein family HMM PF00742; match to protein family HMM PF01842; match to protein family HMM PF03447. | 0.886 |
PFL_4812 | ilvA_1 | PFL_4812 | PFL_3098 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.936 |
PFL_4812 | ilvA_2 | PFL_4812 | PFL_5905 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Threonine ammonia-lyase, biosynthetic; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. | 0.925 |
PFL_4812 | ilvC | PFL_4812 | PFL_5253 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | Ketol-acid reductoisomerase; Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate. | 0.984 |
PFL_4812 | leuA | PFL_4812 | PFL_4946 | Putative thiamine pyrophosphate enzyme; Identified by match to protein family HMM PF00205; match to protein family HMM PF02775; match to protein family HMM PF02776; Belongs to the TPP enzyme family. | 2-isopropylmalate synthase; Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate); Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily. | 0.897 |