Rv1557, (MTCY48.08c), len: 397 aa. Probable mmpL6,conserved transmembrane transport protein (see citations below). Member of RND superfamily, with strong similarity to C-terminal part of members of large Mycobacterial membrane protein family belonging to RND superfamily including: mmpL1, mmpL2, mmpL3, etc. Probably truncated (see Brosch et al., 2002). Belongs to the MmpL family.

Probable threonine dehydratase IlvA; Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2- ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity).

Probable F420 biosynthesis protein FbiA; Catalyzes the transfer of the phosphoenolpyruvate moiety from enoylpyruvoyl-2-diphospho-5'-guanosine (EPPG) to 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO) with the formation of dehydro coenzyme F420-0 and GMP; Belongs to the CofD family.

F420H(2)-dependent reductase Rv1155; F420H(2)-dependent reductase able to catalyze the reduction of biliverdin-IXalpha to bilirubin-IXalpha in vitro. However, kinetic parameters show that it is less efficient than the biliverdin reductase Rv2074 and suggest biliverdin-IXalpha is unlikely to be the native substrate of Rv1155, which probably catalyzes the reduction of an alternative molecule in vivo. Binds coenzyme F420, but does not bind FMN or other flavins. Cannot use pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate, pyridoxal 5'- phosphate (PLP), the anti-tuberculosis drug PA-824 o [...]

Probable F420 biosynthesis protein FbiB; Bifunctional enzyme that catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L- lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2- dependent reduction of dehydro-F420-0 to form F420-0.

Conserved hypothetical alanine rich protein; Guanylyltransferase that catalyzes the activation of phosphoenolpyruvate (PEP) as enolpyruvoyl-2-diphospho-5'-guanosine, via the condensation of PEP with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.

Rv0044c, (MTCY21D4.07c), len: 264 aa. Possible oxidoreductase, highly similar to AAD32732.1|MmcI|AF127374| F420-dependent H4MPT reductase from Streptomyces lavendulae (264 aa). Also similar to Mycobacterium tuberculosis proteins e.g. Rv1855c, Rv0953c, Rv0791c, Rv0132c, etc.

Rv3520c, (MTV023.27c), len: 347 aa. Possible coenzyme F420-dependent oxidoreductase, equivalent to Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 2029, E(): 9.1e-120, (86.85% identity in 342 aa overlap). Similar to many coenzyme F420-dependent enzymes (and other proteins) e.g. Q9AD98|SCI52.11c putative ATP/GTP-binding protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 859, E(): 1.6e-46, (41.9% identity in 346 aa overlap); Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), FA [...]

Possible oxidoreductase; Catalyzes the reduction of the keto moiety of phthiodiolone dimycocerosates (DIM B) and glycosylated phenolphthiodiolone dimycocerosates to form the intermediate compounds phthiotriol and glycosylated phenolphthiotriol dimycocerosates during phthiocerol dimycocerosates (DIM A) and glycosylated phenolphthiocerol dimycocerosates (PGL) biosynthesis.