Long (C50) chain Z-isoprenyl diphosphate synthase (Z-decaprenyl diphosphate synthase); Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) in the cis configuration with (2Z,6E)-farnesyl diphosphate (Z-FPP or EZ-FPP) generating the 50 carbon product trans,polycis-decaprenyl diphosphate. When (2E,6E)-farnesyl diphosphate (E-FPP or EE-FPP) is used in vitro, both primary products decaprenyl diphosphate and (2E,6E,10E)-geranylgeranyl diphosphate (EEE-GGPP) are synthesized. M.tuberculosis does not synthesize (2E,6E,10Z)- geranylgeranyl diphosphate (EEZ-GGPP) and heptapreny [...]

Unknown protein; Rv2360c, (MTCY27.20), len: 142 aa. Unknown protein.

RecBCD enzyme subunit RecB; A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holo [...]

Probable DNA repair protein RecN (recombination protein N); May be involved in recombinational repair of damaged DNA.

RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.

Rv2363, (MTCY27.17c), len: 484 aa. Probable amiA2,amidase, highly similar or similar to others e.g. O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 777,E(): 1.1e-38, (35.0% identity in 474 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 770, E(): 3e-38, (36.4% identity in 456 aa overlap); Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 701, E(): 3.5e-34, (32.7% identity in 468 aa overlap); etc. Also highly similar t [...]

Probable holliday junction DNA helicase RuvB; The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.

Rv2368c, (MTCY27.12), len: 352 aa. Probable phoH1,phoH-like protein (phosphate starvation-induced protein),probably ATP-binding protein, equivalent to Q49751|PHOL_MYCLE| ML0627|B1937_F1_20 PHOH-like protein from Mycobacterium leprae (349 aa), FASTA scores: opt: 1952, E(): 4.7e-107, (88.9% identity in 352 aa overlap). Also highly similar to Q9L2L5|SCC117.05 PHOH-like protein from Streptomyces coelicolor (359 aa), FASTA scores: opt: 1407, E(): 3.6e-75, (63.6% identity in 349 aa overlap); Q9RSY1|DR1988 PHOH-related protein from Deinococcus radiodurans (380 aa), FASTA scores: opt: 1053, E( [...]

Probable adenine glycosylase MutY; Adenine glycosylase active on G:A and C:A mispairs, as well as processing 7,8-dihydro-8-oxoguanine:A (8-oxoG) mismatches. Minor activity against 8-oxoG:G and 8-oxo:T mismatches is also seen. Bind dsDNA oligonucleotides containing the above mismatches.