Trehalose synthase TreS; Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D- glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Might also function as a sensor and/or regulator of trehalose levels [...]

Maltooligosyltrehalose trehalohydrolase TreZ; Is involved in the biosynthesis of trehalose but not in that of capsular glucan and glycogen.

Rv2006, (MTCY39.11c), len: 1327 aa. OtsB1,trehalose-6-phosphate phosphatase (see citations below). Belongs to Glycosyl hydrolases family 65. Note that previously known as otsB. Predicted possible vaccine candidate (See Zvi et al., 2008).

Trehalose-6-phosphate synthase; Involved in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1- phosphate (M1P), and probably in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6- phosphate (Glc-6-P) to form trehalose-6-phosphate. Is specific for the glucosyl acceptor (Glc-6-P cannot be replaced by either mannose-6-P, fructose-6-P or glucosamine-6-P), but any of the glucose sugar nucleotides can be used as glucosyl donors. It is more active with th [...]

Conserved protein; Rv3401, (MTCY78.27c), len: 786 aa. Conserved protein, may be an hydrolase or a transferase, equivalent to Q49736|ML0392|B1620_F1_30 hypothetical 88.1 KDA protein from Mycobacterium leprae (792 aa), FASTA scores: opt: 4820, E(): 0, (91.45% identity in 782 aa overlap). Also highly similar to Q9L2I8|SCF42.31c putative glycosyl transferase from Streptomyces coelicolor (792 aa), FASTA scores: opt: 3060, E(): 2.9e-179, (59.25% identity in 785 aa overlap); and similar to others e.g. Q9K109|NMB0390 maltose phosphorylase from Neisseria meningitidis (serogroup B) (752 aa), FAS [...]

Maltooligosyltrehalose synthase TreY; Catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-D-glucoside) by intramolecular transglycosylation.

Rv2401A, len: 67 aa. Possible conserved membrane protein, highly similar, but with 29 aa shorter, to ML0614|AL583919_34|Q49760 from Mycobacterium leprae (95 aa), FASTA scores: opt: 297, E(): 3.6e-15, (67.7% identity in 65 aa overlap). Has hydrophobic stretch.

Probable glucanase GlgE; Essential maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)- glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP5 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Exhibits an alpha-retaining catalytic mechanism. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen in the presence [...]

Maltokinase Mak; Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate (By similarity). Is involved in a branched alpha- glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB; Belongs to the aminoglycoside phosphotransferase family.