Rv3394c, (MTV004.52c), len: 527 aa. Hypothetical protein, with some similarity to various bacterial proteins e.g. BAB51085|MLR4427 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (545 aa), FASTA scores: opt: 267,E(): 2.8e-08, (26.5% identity in 509 aa overlap); BAB48362|MLR0866 DNA damage inducible protein P from Rhizobium loti (Mesorhizobium loti) (438 aa), FASTA scores: opt: 245, E(): 4.6e-07, (25.5% identity in 290 aa overlap); Q9S292|SCI11.27c hypothetical protein from Streptomyces coelicolor (322 aa), FASTA scores: opt: 202, E(): 0.00012,(28.5% identity in 323 aa ove [...]

DNA polymerase III (beta chain) DnaN (DNA nucleotidyltransferase); Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP- independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta ch [...]

Rv2413c, (MTCY253.07), len: 316 aa. Conserved hypothetical protein, highly similar to O33133|MLCL536.07c|ML0603|Q49756|G466975|B1937_F2_36 hypothetical 39.1 KDA protein from Mycobacterium leprae (389 aa), FASTA scores: opt: 1683, E(): 1.8e-88, (83.9% identity in 316 aa overlap). ML0603 is a putative lipoprotein with an N-terminal signal sequence and appropriately positioned prokaryotic lipoprotein lipid attachment site that is not present in Rv2413c as this seems to be 73 aa shorter. Also some similarity with various proteins from other organisms e.g. Q9RDM2|SCC123.02c putative DNA-bin [...]

RecA protein (recombinase A) [contains: endonuclease PI-MTUI (MTU RecA intein)]; Can catalyze the hydrolysis of ATP in the presence of single- stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.

Rv2191, (MTCY190.02), len: 645 aa. Conserved hypothetical protein, similar to SW:DP3A_B ACSU P13267 DNA polymerase III, alpha chain (31.3% identity in 249 aa overlap) and SW:UVRC_ECOLI P07028 excinuclease ABC subunit C (25.7% identity in 230 aa overlap). Also similar to M. tuberculosis Rv3711c (dnaQ DNA polymerase III e chain) and Rv1420 (uvrC excinuclease ABC subunit C).

Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)); Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).

Repressor LexA; Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Has been shown to bind to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.

Rv0054, (MTCY21D4.17), len: 164 aa. ssb,single-strand binding protein (see Mizrahi & Andersen 1998), highly similar to others. Belongs to the SSB family.

Rv2478c, (MTV008.34c), len: 161 aa. Conserved hypothetical protein, with weak similarity with many single-strand binding proteins e.g. Q9X8U3|SCH24.29 putative single-strand binding protein from Streptomyces coelicolor (199 aa), FASTA scores: opt: 246, E(): 4.5e-08,(31.5% identity in 162 aa overlap); P46390|SSB_MYCLE|ML2684|MLCB1913.20c single-strand binding protein (SSB) (helix-destabilizing protein) from Mycobacterium leprae (168 aa), FASTA scores: opt: 239, E(): 1e-07, (30.8% identity in 146 aa overlap); P18310|SSBF_ECOLI single-strand binding protein from Escherichia coli (178 aa), [...]