Rv0331, (MTCY63.36), len: 388 aa. Possible dehydrogenase/reductase, similar to various dehydrogenases/reductases e.g. NP_103779.1|14022957|BAB49565.1|AP002999 flavoprotein reductase from Mesorhizobium loti (377 aa); NP_147681.1 predicted NAD(FAD)-dependent dehydrogenase from Aeropyrum pernix (381 aa); DHSU_CHRVI|Q06530 sulfide dehydrogenase (431 aa), FASTA scores: opt: 347, E(): 6.8e-15, (25.6% identity in 348 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al.,2007).

Conserved protein; In vitro, the reduced form of Rv2466c catalyzes the reduction and activation of TP053, which is a thienopyrimidine derivative drug that can kill both replicating and non-replicating M.tuberculosis.

dTDP-glucose 4,6-dehydratase RmlB; Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6- deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D- N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.

dTDP-4-dehydrorhamnose 3,5-epimerase; Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.

Rv1471, (MTV007.18), len: 123 aa. Probable trxB1,thioredoxin, similar to many bacterial thioredoxins e.g. P33636|THI2_ECOLI from Escherichia coli (139 aa), FASTA scores: opt: 290, E(): 1.8e-13, (44.3% identity in 97 aa overlap); etc. Highly similar to Rv1470|TrxA probable thioredoxin from Mycobacterium tuberculosis (124 aa), FASTA scores: opt: 402, E(): 1.2e-32, (54.4% identity in 114 aa overlap). Contains PS00194 Thioredoxin family active site. Belongs to the thioredoxin family. Note that previously known as trxB.

Probable translation initiation factor if-1 InfA; One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.

Rv3368c, (MTV004.26c), len: 214 aa. Possible oxidoreductase, equivalent to O07697|MLCL383.23|ML0418 hypothetical 23.6 KDA protein (putative oxidoreductase) from Mycobacterium leprae (210 aa), FASTA scores: opt: 1215, E(): 1.5e-74, (81.4% identity in 210 aa overlap). Also similar to O30106|AF0131 putative NAD(P)H-flavin oxidoreductase from Archaeoglobus fulgidus (194 aa), FASTA scores: opt: 139, E(): 0.028, (29.0% identity in 207 aa overlap); Q60049|NOX_THETH NADH dehydrogenase from Thermus aquaticus (subsp. thermophilus) (205 aa), FASTA scores: opt: 169, E(): 0.00028, (28.3% identity i [...]

Conserved protein; Rv1875, (MTCY180.43c), len: 147 aa. Conserved protein. Some similarity to Mycobacterium tuberculosis hypothetical proteins e.g. Rv1155|MTCI65.22|Z95584 (147 aa), FASTA scores: opt: 178, E(): 7.4e-06, (26.9% identity in 130 aa overlap); Rv0121c and Rv2074. Also similar to AL079356|SC6G9.21 hypothetical protein from Streptomyces coelicolor (144 aa), FASTA scores: opt: 239, E(): 3.1 e-09,(38.7% identity in 137 aa overlap).

F420H(2)-dependent reductase Rv1155; F420H(2)-dependent reductase able to catalyze the reduction of biliverdin-IXalpha to bilirubin-IXalpha in vitro. However, kinetic parameters show that it is less efficient than the biliverdin reductase Rv2074 and suggest biliverdin-IXalpha is unlikely to be the native substrate of Rv1155, which probably catalyzes the reduction of an alternative molecule in vivo. Binds coenzyme F420, but does not bind FMN or other flavins. Cannot use pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate, pyridoxal 5'- phosphate (PLP), the anti-tuberculosis drug PA-824 o [...]