node1 | node2 | node1 accession | node2 accession | node1 annotation | node2 annotation | score |
Rv1428c | echA1 | Rv1428c | Rv0222 | Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355,E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. | Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. | 0.578 |
Rv1428c | nat | Rv1428c | Rv3566c | Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355,E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.632 |
Rv1498A | hsaD | Rv1498A | Rv3569c | Rv1498A, len: 70 aa. Conserved protein, highly similar to other hypothetical proteins e.g. from Streptomyces coelicolor, Sinorhizobium meliloti and Pseudomonas aeruginosa. | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.430 |
Rv1498A | nat | Rv1498A | Rv3566c | Rv1498A, len: 70 aa. Conserved protein, highly similar to other hypothetical proteins e.g. from Streptomyces coelicolor, Sinorhizobium meliloti and Pseudomonas aeruginosa. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.641 |
Rv2669 | nat | Rv2669 | Rv3566c | GCN5-related N-acetyltransferase; Rv2669, (MTCY441.38), len: 156 aa. Probable acetyltransferase. Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Similarity to several proteins e.g. Q9A6M0|CC2073 acetyltransferase (GNAT family) from Caulobacter crescentus (178 aa), FASTA scores: opt: 242, E(): 1.2e-09, (30.9% identity in 165 aa overlap); Q99RQ8|SA2159 hypothetical protein similar to transcription repressor of sporulation,septation and degradation paiA from Staphylococcus aureus subsp. aureus N315 (171 aa), FASTA scores: opt: 214, E(): 9.8e-08, (27.5% id [...] | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.661 |
Rv3566A | hsaB | Rv3566A | Rv3567c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 0.826 |
Rv3566A | hsaC | Rv3566A | Rv3568c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.475 |
Rv3566A | hsaD | Rv3566A | Rv3569c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.414 |
Rv3566A | nat | Rv3566A | Rv3566c | Rv3566A, len: 88 aa. Hypothetical unknown protein. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.938 |
echA1 | Rv1428c | Rv0222 | Rv1428c | Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. | Rv1428c, (MTCY493.26), len: 275 aa. Conserved hypothetical protein, some similarity to hypothetical proteins from Mycobacterium tuberculosis e.g. Rv0502|YV29_MYCTU|Q11167 (358 aa), FASTA scores: opt: 355,E(): 5e-16, (32.6% identity in 273 aa overlap); and Rv1920. | 0.578 |
echA1 | hsaA | Rv0222 | Rv3570c | Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 0.588 |
echA1 | nat | Rv0222 | Rv3566c | Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.545 |
hsaA | echA1 | Rv3570c | Rv0222 | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase); Rv0222, (MTCY08D5.17), len: 262 aa. Probable echA1,enoyl-CoA hydratase, similar to others e.g. AAC77915.1|AF063588 enoyl CoA hydratase from Rhodococcus fascians (275 aa); etc. | 0.588 |
hsaA | hsaB | Rv3570c | Rv3567c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 0.999 |
hsaA | hsaC | Rv3570c | Rv3568c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.999 |
hsaA | hsaD | Rv3570c | Rv3569c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 4,9-DHSA hydrolase; Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9- DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5- methyl-6-oxoocta-2,4-dienoic acid (HOPODA). Belongs to the AB hydrolase superfamily. HsaD family. | 0.999 |
hsaA | nat | Rv3570c | Rv3566c | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | Arylamine N-acetyltransferase Nat (arylamine acetylase); Catalyzes the transfer of the acetyl group from acetyl coenzyme A to the free amino group of arylamines and hydrazines. Is able to utilize not only acetyl-CoA, but also n- propionyl-CoA and acetoacetyl-CoA as acyl donors, although at a lower rate. As acetyl-CoA and propionyl-CoA are products of cholesterol catabolism and the nat gene is likely present in the same operon than genes involved in cholesterol degradation, this enzyme could have a role in the utilization and regulation of these CoA species. | 0.697 |
hsaB | Rv3566A | Rv3567c | Rv3566A | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | Rv3566A, len: 88 aa. Hypothetical unknown protein. | 0.826 |
hsaB | hsaA | Rv3567c | Rv3570c | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | Flavin-dependent monooxygenase, oxygenase subunit HsaA; Catalyzes the o-hydroxylation of 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3-HSA) to 3,4-dihydroxy-9,10-secoandrosta- 1,3,5(10)-triene-9,17-dione (3,4-DHSA) in the catabolism of cholesterol. Can use either FADH(2) or FMNH(2) as flavin cosubstrate. Also catalyzes the o-hydroxylation of a range of p-substituted phenols to generate the corresponding catechols. | 0.999 |
hsaB | hsaC | Rv3567c | Rv3568c | Flavin-dependent monooxygenase, reductase subunit HsaB; Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit; Belongs to the non-flavoprotein flavin reductase family. | 3,4-DHSA dioxygenase; Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5- 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA). | 0.999 |