Rv3606c, (MTCY07H7B.16), len: 188 aa. Probable folK,2-amino-4-hydroxy-6-hydroxymethyldihydropterine pyrophosphokinase, equivalent to O69528|HPPK_MYCLE|folk|ML0226MLCB2548.05c 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase from Mycobacterium leprae (191 aa) FASTA scores: opt: 772, E(): 1.2e-44, (63.15% identity in 190 aa overlap). Also similar to many e.g. P71512|HPPK_METEX|folk|FOLA from Methylobacterium extorquens (158 aa), FASTA scores: opt: 292, E(): 1.4e-12,(36.85% identity in 171 aa overlap); O33726|HPPK_STRPY|folk|SPY1100 from Streptococcus pyogenes (166 aa) [...]

Probable dihydroneopterin aldolase FolB (DHNA); Catalyzes the conversion of 7,8-dihydroneopterin to 6- hydroxymethyl-7,8-dihydropterin, 7,8-dihydromonapterin and 7,8- dihydroxanthopterin, respectively, in equal quantities. After longer incubation times the only product is 6-hydroxymethyl-7,8-dihydropterin.

GTP cyclohydrolase I FolE (GTP-ch-I); Rv3609c, (MTCY07H7B.13), len: 202 aa. Probable folE (alternate gene name: gchA), GTP cyclohydrolase I,equivalent to O69531|GCH1_MYCLE|FOLE|ML0223|MLCB2548.08c GTP cyclohydrolase I from Mycobacterium leprae (205 aa) FASTA scores: opt: 1112, E(): 3.8e-63, (81.95% identity in 205 aa overlap). Also highly similar to many e.g. Q9X8I3|GCH1_STRCO|FOLE|SCE9.10c from Streptomyces coelicolor (201 aa), FASTA scores: opt: 873, E(): 4.2e-48,(67.4% identity in 187 aa overlap); Q9KCC7|MTRA|BH1646 from Bacillus halodurans (188 aa), FASTA scores: opt: 757, E(): 8.1 [...]

Membrane-bound protease FtsH (cell division protein); Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.

Dihydropteroate synthase 2 FolP2 (DHPS 2) (dihydropteroate pyrophosphorylase 2); Has very low affinity for the DHPS substrate 6-hydroxymethyl- 7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism.

Rv0812, (MTV043.04), len: 289 aa. Probable amino acid aminotransferase, similar to other amino acid aminotransferases, generelly class-IV of pyridoxal-phosphate-dependent aminotransferases, and especially ILVE proteins and PABC proteins e.g. B76065.1|AL157953 putative aminotransferase from Streptomyces coelicolor (273 aa); NP_069766.1|NC_000917 branched-chain amino acid aminotransferase (ilvE) from Archaeoglobus fulgidus (290 aa); P54692|DAAA_BACLI D-alanine aminotransferase from Bacillus licheniformis (283 aa); P28305|PABC_ECOLI|B1096 4-amino-4-deoxychorismate lyase (ADC lyase) From E [...]

Rv3605c, (MTCY07H7B.17), len: 158 aa. Probable conserved secreted or membrane protein, identical to O69527|MLCB2548.04c|ML0227 putative membrane protein from Mycobacterium leprae (158 aa), FASTA scores: opt: 944, E(): 2.6e-56, (85.45% identity in 158 aa overlap). Also similar to other proteins e.g. Q9X8I2|SCE9.09 possible secreted protein from Streptomyces coelicolor (162 aa), FASTA scores: opt: 174, E(): 9.2e-05, (31.25% identity in 128 aa overlap); etc. Contains possible N-terminal signal sequence. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004).

Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase); Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis; Belongs to the dihydrofolate reductase family.

Probable thymidylate synthase ThyA (ts) (TSASE); Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.